![]() ![]() When transcript and CDS pairs are merged, the coding exons are rendered in dark green, and non-coding regions (i.e. When all features are expanded, gene bars are colored green, non-coding RNAs and mRNA features are colored purple, and CDS features are colored red. Track display settings include multiple rendering options as described below. You may also have options to show only variants that are part of the CCDS project or have been designated as the RefSeq or MANE Select variant. The Hide model transcripts feature hides transcript models predicted using NCBI's Gnomon algorithm. The option Hide non-coding will hide non-coding transcripts or non-coding genes or pseudogenes from view, so that only protein-coding transcript variants are displayed. Several options may be available for NCBI or Ensembl gene annotation tracks. The track display settings dialog can be accessed by clicking on the track title, the gear icon on the right side of the track, or by selecting the track within the Tracks configuration menu. Gene model features are comprised of multiple possible components: gene bar, RNA/mRNA, CDS, and exon features. Square for feature start, arrow for feature stop, dashed lines for intronsĬircle for feature start, arrow for feature stop, dashed lines for intronsĬircle for mRNA start only, square for other features start except for gene and CDS, arrow for feature stop, shaded bars for mRNA introns, and curvy lines for CDS introns Solid bars for feature intervals or exons, and solid lines for intronsĪrrows at both ends showing the strand, and shaded bars for introns No triangles hairlines mark feature boundaries and cut sites Restriction site (feature selected, zoomed out) Triangles indicate cut site hairlines mark feature boundaries and cut sites ![]() When the feature is selected using a mouse click, the location of the cut sites is indicated by red hairlines. Triangle marks are not present at high zoom levels. Restriction enzyme recognition sites are rendered with triangle marks indicating the cut sites. White arrows (">") at both ends or black arrows (">") at one end. due to assembly errors or gaps)įeature marked as partial at 5’ and 3’ ends (white arrows, ">") and feature with a partial 3' end (black arrows ">") RNA or CDS feature with sequence discrepancy relative to the assemblyġ.3.3 Features with partial annotation location (e.g. Show on single line with exon structure, no gene barġ.3.2 Features with imperfect match to the assembly 1.3 Special Rendering Styles 1.3.1 Pseudogene features Users can also simplify this track display by filtering the data based on the number of spliced reads. Intron features tracks contain additional track display options, including the ability to sort splice junction features based on the number of spliced reads or by the splice direction (strand). Hover over the feature to see a tooltip that provides additional information, including the end coordinates and intron length. The number of RNA-seq reads supporting the splice junction is reported for each feature. The ends of the features correspond to splice junctions, while the arrow corresponds to the direction of splicing. Intron feature tracks display exon-exon splice junctions based on an analysis of RNA-seq data. Changing these options may result in showing more or fewer features in the track, depending on the underlying source of data in the track. "Show all" "Show parent, Merge children" "Show children, not parent" "Show parent, not children" "Superimpose children on parent". The Feature linking options provide different display views for tracks containing parent and child features i.e. The option "All features on a single line" alway shows the data as a histogram, while the option "All features are expanded" always shows discrete features. By default, most feature tracks are set to "Pack features if necessary", where individual features are shown when zoomed in and the data is displayed as a histogram when zoomed out. Users can set the display resolution using the Rendering Options dropdown menu. in RNA and coding region features) indicate the direction of splicing. White arrows inside features indicate the direction of the feature relative to the top-level sequence. Protein feature or other annotated feature Specialized rendering for SNPs, structural variants, and segmental duplications are described in the later sections of this documentation. These features often derive from files formatted as BED, bigBED, GFF3, GTF, ASN.1, and similar formats. This section describes the rendering used for gene features, RNA and protein models, regulatory sites, and most other feature types. The NCBI Genome Workbench uses similar graphical renderings. This legend applies to NCBI Sequence Viewer. ![]()
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